Run Seurat MapQuery to reference

This will normalize the query object, find transfer anchors, and map the query to the reference.

Usage

RunSeuratMap2Ref(
  object,
  ref,
  use,
  ident = "seurat_clusters",
  refnorm = c("auto", "LogNormalize", "SCT", "SCTransform"),
  skip_if_normalized = TRUE,
  split_by = NULL,
  ncores = 1,
  MapQuery_args = list(refdata = list()),
  FindTransferAnchors_args = list(),
  SCTransform_args = list(),
  NormalizeData_args = list(),
  log = NULL,
  cache = NULL
)

Arguments

object

Seurat object

ref

Reference Seurat object or a file path to a Seurat object with .rds or .h5seurat extension

use

The name in the metadata of the reference to use as identity for the query after mapping

ident

The name of the identity in metadata after mapping

refnorm

The normalization method used by the reference. The same normalization method will be used for the query.

"auto": automatically detect the normalization method used by the reference
"LogNormalize": LogNormalize
"SCT": SCTransform
"SCTransform": SCTransform (alias for SCT)

skip_if_normalized

Skip normalization if the query is already normalized with the same method as the reference

split_by

The name of the metadata to split the query object by and do the mapping separately

ncores

Number of cores to use for parallel processing for the split query objects

MapQuery_args

Arguments to pass to Seurat::MapQuery(). The use argument will be added to the refdata list.

FindTransferAnchors_args

Arguments to pass to Seurat::FindTransferAnchors().

SCTransform_args

Arguments to pass to Seurat::SCTransform(). Will be used to normalize the query object if refnorm is set to "SCT"

NormalizeData_args

Arguments to pass to Seurat::NormalizeData(). Will be used to normalize the query object if refnorm is set to "LogNormalize"

log

Logger

cache

Directory to cache the results. Set to FALSE to disable caching

Value

The Seurat object with the mapped data

Usage

Arguments

Value

See also