Run DoubletFinder on a Seurat object

Run DoubletFinder on a Seurat object

Usage

RunSeuratDoubletFinder(
  object,
  ncores = 1,
  PCs = 30,
  pN = 0.25,
  doublets = 0.075,
  log = NULL,
  allow_warnings = FALSE
)

Arguments

object

Seurat object

ncores

Number of cores to use

PCs

Number of statistically-significant principal components

pN

The number of generated artificial doublets, expressed as a proportion of the merged real-artificial data. Default is set to 0.25, based on observation that DoubletFinder performance is largely pN-invariant (see McGinnis, Murrow and Gartner 2019, Cell Systems).

doublets

The expected proportion of doublets in the dataset. Default is set to 0.075

Value

The Seurat object with doublet detection results in @misc$doubletFinder