Run DoubletFinder on a Seurat object
Usage
RunSeuratDoubletFinder(
object,
ncores = 1,
PCs = 30,
pN = 0.25,
doublets = 0.075,
log = NULL,
allow_warnings = FALSE
)Arguments
- object
Seurat object
- ncores
Number of cores to use
- PCs
Number of statistically-significant principal components
- pN
The number of generated artificial doublets, expressed as a proportion of the merged real-artificial data. Default is set to 0.25, based on observation that DoubletFinder performance is largely pN-invariant (see McGinnis, Murrow and Gartner 2019, Cell Systems).
- doublets
The expected proportion of doublets in the dataset. Default is set to 0.075